Laboratory Safety - Chemical Hygiene Program
CHEMICAL HYGIENE PROGRAM
Printable files are available in three parts with Adobe Acrobat Reader:
- PDF Version of Chemical Hygiene Program
- PDF Version of Appendices 1 through 11
- PDF Version of Appendix 12 - Standard Operating Procedures
OSHA and EPA regulations require all chemical containers be labeled. To assist lab personnel in the identification of laboratory prepared solutions, the following abbreviations were approved by the Departmental Chemical Hygiene Officer Committee. The hazards are based on the pure materials. The chemicals may pose a lesser hazard when they are diluted with water.
The National Fire Protection Association (NFPA) rates the hazard of a chemical on a scale of 0 to 4 (0 is no hazard and 4 is severe hazard). The hazards include:
- H = health hazard
- F = fire hazard
- R = reactivity
- Special hazards (example, OX = oxidizing hazard).
|
CHEMICAL/SOLUTION NAME |
ABBREVIATION |
HAZARD WARNING |
NFPA RATING |
|
5-Bromo-4-Chloro-3-Indolyl Beta-D-Galactopyranoside |
X-gal |
Irritation to eyes/skin/respiratory tract/digestive tract |
H1 F1 R0 |
|
Acetic Acid |
CH3COOH |
Corrosive. Irritation to eyes/skin/respiratory tract |
H3 F2 R0 |
|
Amino Ethyl Carbazole |
AEC |
Carcinogen. Irritation to eyes/skin/respiratory tract |
H2 F1 R0 |
|
Ammonium Acetate |
NH4OAC |
Irritation to eyes/skin/respiratory tract |
H1 F1 R1 |
|
Ammonium Bicarbonate |
NH4HCO3 |
Irritation to eyes/skin |
H1 F0 R0 |
|
Ammonium Chloride |
NH4Cl |
Irritation to eyes/skin |
H2 F0 R0 |
|
Ammonium Hydroxide |
NH4OH |
Corrosive to eyes/skin |
H3 F1 R0 |
|
Ampicillin |
Amp |
Antibiotic |
---- |
|
Beta-Mercaptoethanol, 2-Mercaptoethanol |
BME |
Flammable. Severe irritation to eyes/skin/respiratory tract |
H3 F2 R1 |
|
Bleach (10% diluted solution) |
10% bleach |
Irritation to eyes/skin |
H1 F0 R1 |
|
Bovine Serum Albumin |
BSA |
H0 F0 R0 |
|
|
Bromphenol Blue |
BPB |
No information available |
H1 F1 R0 |
|
Calcium Chloride |
CaCl2 |
Irritation to eyes/skin/respiratory tract |
H2 F0 R2 |
|
Carbenicillin |
Carb |
Irritation to eyes/skin/respiratory tract. Sensitization. |
H2 F0 R2 |
|
Cesium Chloride |
CsCl |
Irritation to eyes/skin/respiratory tract |
H2 F0 R0 |
|
Chloroform |
CHCl3 |
Carcinogen. Irritation to eyes/skin/respiratory tract |
H2 F0 R0 |
|
Diaminobenzidine |
DAB |
Carcinogen. Irritation to eyes/skin/respiratory tract |
H2 F0 R0 |
|
Dimethylformamide |
DMF |
Irritation to eyes/skin/respiratory tract |
H2 F2 R0 |
|
Dimethyl Sulfoxide |
DMSO |
Irritation to eyes/skin |
H2 F1 R0 |
|
Dulbecco's Phosphate Buffer Saline |
DPBS |
No information available |
H1 F0 R0 |
|
EBC Lysis Buffer |
EBC |
||
|
Ethanol |
EtOH |
Flammable. Irritation to eyes/skin/respirators tract |
H2 F3 R0 |
|
Ethidium Bromide |
EtBr |
Irritation to eyes/skin/respiratory tract |
H2 F1 R0 |
|
Ethylenediaminetetraacetic Acid |
EDTA |
Mild Irritation to eyes/skin/respiratory tract |
H2 F1 R0 |
|
Fetal Bovine Serum |
FBS |
Irritation to eyes/skin/respiratory tract. |
H0 F0 R0 |
|
Glucose-Tris-EDTA |
GTE |
Irritation to eyes/skin/respiratory tract. |
H2 F0 R0 |
|
Hydrochloric Acid |
HCl |
Corrosive. Burns to eyes/skin/respiratory tract |
H3 F0 R1 |
|
Hydrofluoric Acid |
HF |
Corrosive. Burns to eyes/skin/respiratory tract |
H4 F0 R0 |
|
Hydrogen Peroxide |
H2O2 |
Oxidizer. Burns to eyes/skin |
H3 F0 R1 OX |
|
Hygromycin |
Hygro |
Antibiotic |
---- |
|
Isopropyl beta-D-thiogalactopyranoside |
IPTG |
No information available |
H1 F1 R0 |
|
Kanamycin |
Kan |
Antibiotic |
---- |
|
Luria-Bertani Medium |
LB |
H0 F0 R0 |
|
|
M9 Salts |
M9Salts |
H0 F0 R0 |
|
|
M9 Terrific Broth |
M9TB |
H0 F0 R0 |
|
|
M9Luria Broth |
M9LB |
H0 F0 R0 |
|
|
Magnesium Acetate |
MgOAC |
Mild Irritation to eyes/skin/respiratory tract |
H1 F1 R0 |
|
Magnesium Chloride |
MgCl2 |
Irritation to eyes/skin |
H2 F0 R0 |
|
Magnesium Sulfate |
MgSO4 |
Irritation to respiratory tract |
H1 F0 R0 |
|
Methanol |
MeOH |
Flammable. Poison. Irritation to eyes/skin/respiratory tract |
H2 F3 R0 |
|
N,N-Dimethyl Formamide |
DMF |
Flammable. Strong Irritation to eyes/skin/respiratory tract |
H2 F2 R0 |
|
N-2-Hydroxyethylpiperazine-N'-2-ethanesulfonic acid |
HEPES |
No information available |
H1 F1 R0 |
|
Neomycin |
Neo |
Antibiotic |
---- |
|
Phenylmethylsofonyl Fluoride |
PMSF |
Severe Irritation to eyes/skin/respiratory tract |
H3 F1 R2 |
|
Phosphate Buffered Saline |
PBS |
No information available |
H1 F0 R0 |
|
Phosphate Buffered Saline-Tween |
PBST, PBS-Tween |
Irritation to eyes/skin/respiratory tract |
H1 F0 R0 |
|
Piperazine-N, N'-bis[2-ethanesulfonic acid] |
PIPES |
Irritation to eyes/skin/respiratory tract |
H1 F1 R0 |
|
Polyacrylamide Gel Electrophoresis Running Buffer |
PAGE Running Buffer |
Irritation to eyes/skin/respiratory tract |
H1 F0 R0 |
|
Polyacrylamide Gel Electrophoresis Transfer Buffer |
Page Transfer Buffer |
Irritation to eyes/skin/respiratory tract |
H1 F0 R0 |
|
Polyethylene Glycol |
PEG |
No information available |
H1 F1 R0 |
|
Polyvinyl Alcohol |
PVA, PVOH |
Irritation to skin |
H0 F2 R0 |
|
Potassium Acetate |
KOAc |
Mild Irritation to eyes/skin/respiratory tract |
H0 F1 R0 |
|
Potassium Chloride |
KCl |
Mild Irritation to eyes/skin/respiratory tract |
H0 F0 R0 |
|
Potassium Hydroxide |
KOH |
Corrosive. Burns to eyes/skin/respiratory tract |
H3 F0 R1 |
|
Potassium Iodide |
KI |
Irritation to eyes/skin/respiratory tract |
H1 F0 R0 OX |
|
Potassium Phosphate, Dibasic |
K2HPO4 |
Mild Irritation to eyes/skin/respiratory tract |
H1 F0 R0 |
|
Potassium Phosphate, Monobasic |
KH2PO4 |
Mild Irritation to eyes/skin/respiratory tract |
H1 F0 R0 |
|
SOB Medium |
SOB |
No known hazard |
H0 F0 R0 |
|
SOC Medium |
SOC |
No known hazard |
H0 F0 R0 |
|
Sodium Acetate |
NaOAc |
Mild irritation to eyes/skin/respiratory tract |
H0 F1 R0 |
|
Sodium Azide |
NaN3 |
Poison. Irritation to eyes |
H3 F1 R0 |
|
Sodium Bicarbonate |
NaHCO3 |
Irritation to eyes/skin/respiratory tract |
H1 F0 R1 |
|
Sodium Carbonate |
Na2CO3 |
Corrosive. Burns to eyes/skin/respiratory tract |
H3 F0 R1 |
|
Sodium Chloride |
NaCl, Saline |
Irritation to respiratory tract |
H1 F0 R0 |
|
Sodium Citrate |
NaCitrate |
Irritation to eyes/skin/respiratory tract |
H1 F1 R0 |
|
Sodium Dodecyl Sulfate |
SDS |
Irritation to eyes/skin/respiratory tract |
H2 F1 R0 |
|
Sodium Fluoride |
NaF |
Toxic. Irritation to eyes/skin/respiratory tract |
H3 F0 R0 |
|
Sodium Hydroxide |
NaOH |
Corrosive. Burns to eyes/skin/respiratory tract |
H3 F0 R1 |
|
Sodium Lauryl Sulfate |
SLS |
Irritation to eyes/skin/respiratory tract |
H1 F1 R0 |
|
Sodium Phosphate, Monobasic |
NaH2PO4 |
Irritation to eyes/skin/respiratory tract |
H2 F0 R0 |
|
Sodium Phosphate, Dibasic |
Na2HPO4, DSP |
Mild Irritation to eyes/skin/respiratory tract |
H1 F0 R0 |
|
Saline Sodium Citrate |
SSC |
Irritation to eyes/skin/respiratory tract |
H2 F1 R0 |
|
Sulfuric Acid |
H2SO4 |
Corrosive. Burns to eyes/skin/respiratory tract |
H3 F0 R2 |
|
Terrific Broth |
TB |
Irritation to eyes/skin/respiratory tract |
H1 F0 R0 |
|
Tetracycline |
TET |
Antibiotic |
---- |
|
Tris Base [Tris(hydroxymethyl)aminomethane)] |
Tris Base |
Irritation to eyes/skin/respiratory tract |
H2 F0 R0 |
|
Tris Buffered Saline |
TBS |
Irritation to eyes/skin/respiratory tract |
H2 F0 R0 |
|
Tris Buffered Saline-Tween |
TBST, TBS-Tween |
Irritation to eyes/skin/respiratory tract |
H2 F0 R0 |
|
Tris Ethylenediamine-Tetraacetic Acid |
TE, Tris EDTA |
Irritation to eyes/skin/respiratory tract |
H2 F0 R0 |
|
Tris HCL/Acetic Acid/EDTA Buffer |
TAE |
Irritation to eyes/skin/respiratory tract |
H2 F0 R0 |
|
Tris HCL/Boric Acid/EDTA Buffer |
TBE |
Irritation to eyes/skin/respiratory tract |
H2 F1 R0 |
|
Tris Hydrochloride |
Tris-HCl, Tris-Cl |
Irritation to eyes/skin/respiratory tract |
H1 F0 R0 |
|
Tris, Trizma Hydrochloride, |
Tris |
Irritation to eyes/skin/respiratory tract |
H1 F0 R0 |
|
Tris-Acetate-EDTA Buffer |
TAE |
Irritation to eyes/skin/respiratory tract |
H2 F1 R0 |
|
Tris-Borate-EDTA Buffer |
TBE |
Irritation to eyes/skin/respiratory tract |
H2 F1 R0 |
|
Triton (x-100 and x-114) |
TX |
No harmful effects listed |
H1 F1 R0 |
|
Vesphene IISE (working solution) |
Vesphene |
Irritation to eyes/skin |
H1 F0 R0 |
|
Water |
H2O |
None |
H0 F0 R0 |
|
Water, Distilled |
d H2O |
None |
H0 F0 R0 |
|
Water, Double Distilled |
dd H2O |
None |
H0 F0 R0 |
|
Zeocin |
Zeo |
Irritation to eyes/skin/respiratory tract |
H2 F0 R0 |
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This page last updated 2/17/2006. Disclaimer
